MCP-1 activates Syk and Src tyrosine kinases in MonoMac6 cells.

MonoMac6 cells were incubated with 20 nM MCP-1 for the indicated times. Syk (A) and Src (B) kinases were immunoprecipitated (IP) with specific antibodies from cell lysates, resolved by SDS-PAGE, and electrophoretically transferred to Immobilon-P membrane. Syk activity was assessed by immunoblotting using antiphosphotyrosine 4G10 Mabs and Src activity by in vitro kinase assay using enolase as substrate. The amounts of Syk and c-Src immunoprecipitated were evaluated by Western blotting analysis with the appropriate antibodies (WB). The results are representative of 2 experiments.