Fig. 7.
![Fig. 7. MCP-1–induced MonoMac6 cells chemotaxis or transendothelial migration are sensitive to SAPK pharmacologic inhibitors and to PTX. / [3H]-methyl thymidine labeled MonoMac6 cells were washed and pretreated for 2 hours with 40 μM of PD98059, SB203580, or SB202190, 4 hours with 10 μg/mL cholera toxin (CTX) or 16 hours with 100 ng/mL pertussis toxin (PTX) before being tested for their ability to migrate across fibronectin (▩) or TNF-α–activated HUVECs (▨) toward 6 nM MCP-1. Migrated cells, collected after 4 hours in the lower reservoir, were counted by liquid scintillation as described in “Materials and methods.” Results are presented as means ± SD of triplicate samples from 5 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/2/10.1182_blood.v97.2.359/5/h80210590007.jpeg?Expires=1735541857&Signature=z~xZV848pm7jcywqtbZerFRU~KpfM4TZXTiVtRxXn9iDYkUDfedqxbQ9sZisxVcQ1jUiu3kjjjqBE1-Sprlt4iYCsPJ2kiORHDa599lPZoV3~xPaKT7sQiiI8O~wvWGlZ7MKke7CBU2Hk03Q89cJ6wU3bB3Nkz-sjxKOTMsM9BmJp8LkRwP9IpPPv9hMHfjQmUZBe3~D0niZI1Amit6YTpgAT4v~RC9eP7qQ1PwVGU9rTZV3gjxSiCbye-ahN2gAkmYJfOK9FYOUvdlruvlMJEeeRSHs4UVFrCSKI5MsPHze~v-h2z8tEpgcCrg98q1bcOwSpstt8w2BNsD3zCkM6w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
MCP-1–induced MonoMac6 cells chemotaxis or transendothelial migration are sensitive to SAPK pharmacologic inhibitors and to PTX.
[3H]-methyl thymidine labeled MonoMac6 cells were washed and pretreated for 2 hours with 40 μM of PD98059, SB203580, or SB202190, 4 hours with 10 μg/mL cholera toxin (CTX) or 16 hours with 100 ng/mL pertussis toxin (PTX) before being tested for their ability to migrate across fibronectin (▩) or TNF-α–activated HUVECs (▨) toward 6 nM MCP-1. Migrated cells, collected after 4 hours in the lower reservoir, were counted by liquid scintillation as described in “Materials and methods.” Results are presented as means ± SD of triplicate samples from 5 separate experiments.
