Flow cytometric analysis and transplantation results.

(A) Expression of c-Kit and CD34 on Lin⁻ BM cells of a neonatal mouse (Ly-5.2). Lin⁻c-Kit⁺ cells were fractionated into CD34⁻ (R1), CD34^low (R2), and CD34^high (R3) cells on the basis of CD34 expression. The averages of the proportions of the 3 fractions in Lin⁻c-Kit⁺ cells (n = 4) are shown in each window. (B) The percentages of Ly-5.2⁺ cells in B220⁺ cells (B), Thy-1⁺ cells (T), and Gr-1/Mac-1⁺ cells (M) in Ly-5.1 recipient PB 8 to 10 weeks after transplantation. The recipients were transplanted with CD34⁻ (R1), CD34^low (R2), and CD34^high (R3) cells (1 × 10² [●] 1 × 10³ [○] and 5 × 10³ cells [▪]) in BM of neonatal mice (Ly-5.2). (C) A representative PB profile of a recipient mouse (Ly-5.1) engrafted with 1 × 10³Lin⁻c-Kit⁺CD34^high cells (R3) sorted from Ly-5.2 mouse BM. Ly-5.2⁺ cells were present in all of B220⁺ cells, Thy-1⁺ cells, and Gr-1/Mac-1⁺ cells in the PB.