Effect of CXC chemokines on the chemotaxis of NK cells.

In panel A, IL-8 (100 ng/mL), GRO-α (50 ng/mL), IP-10 (25 ng/mL for nonactivated and NA and 1 ng/mL for AD cells), SDF-1α (1 ng/mL), or fractalkine (1 ng/mL for nonactivated and NA and 100 pg/mL for AD cells), was placed in the lower chambers, whereas cells (4 × 10⁵) were placed in the upper wells of Boyden chambers. In panel B, cells (5 × 10⁶/mL) were either untreated or pretreated with 10 μg/mL of either mouse IgG (MIgG) or monoclonal anti-CXCR2 for 1 hour at 4°C. The cells were extensively washed and then placed (4 × 10⁵) in the upper wells, whereas 50 ng/mL of GRO-α was placed in the lower wells of the chambers. In panel C, cells (5 × 10⁶/mL) were either left untreated or were pretreated with 10 μg/mL of rabbit IgG (RIgG) or rabbit anti-CX₃CR1 for 1 hour at 4°C, washed extensively, and then placed (4 × 10⁵) in the upper wells. In the lower wells, fractalkine (1 ng/mL for nonactivated and NA, and 0.1 ng/mL for AD cells) was added. Migration index was calculated by dividing the number of cells migrating in the presence of chemokines by those migrating toward medium only (control: white columns).