Effect of CC chemokines on the in vitro motility of various preparations of NK cells.

In panel A, MCP-1 (25 ng/mL for nonactivated [▩] and NA [▧] and 100 pg/mL for AD [▪] cells), Eotaxin (10 ng/mL for nonactivated and NA and 1 ng/mL for AD cells), MDC (10 ng/mL for nonactivated and NA and 1 ng/mL for AD cells), MIP-3α (25 ng/mL for nonactivated and NA and 1 ng/mL for AD cells), MIP-3β (1 ng/mL for nonactivated, NA, and AD cells), or I-309 (10 ng/mL for nonactivated and NA and 1 ng/mL for AD cells) was placed in the lower wells of Boyden chambers, whereas 4 × 10⁵ (nonactivated, AD, or NA) NK cells were added to the upper wells. In panel B, various concentrations (1 pg/mL, ░; 10 pg/mL, ▩; 100 pg/mL, ▪; 1000 pg/mL, ▤; 10 000 pg/mL, ▥) of vMIP-I were examined. Migration index was calculated as the number of cells migrating in the presence of chemokines divided by the number of cells migrating in the presence of medium only (controls, shown in white columns). Mean ± SD of 6 to 9 experiments. Panel C shows competition binding analysis of 100 pmol/L ¹²⁵I-309 to AD cells, by cold I-309, TARC, or vMIP-I. Log concentrations of these chemokines are shown in the X-axis, whereas the total count of binding is shown in the Y-axis. IC₅₀ for cold ligands is also shown.