Fig. 4.
Fig. 4. IL-10 + TGF-β treatment prevents down-regulation of p27kip1 in IL-10 + TGF-β–tolerant cells. / (A) Lysates from unstimulated, tolerant, and primed T cells were analyzed by SDS-PAGE and immunoblotted with mAb specific for p27kip1. Blots were subsequently stripped and reblotted with mAb specific for p21cip1. (B) Immunoprecipitations were performed with cyclin D2–specific antibody followed by immunoblot with a monoclonal antibody specific for p27kip1.

IL-10 + TGF-β treatment prevents down-regulation of p27kip1 in IL-10 + TGF-β–tolerant cells.

(A) Lysates from unstimulated, tolerant, and primed T cells were analyzed by SDS-PAGE and immunoblotted with mAb specific for p27kip1. Blots were subsequently stripped and reblotted with mAb specific for p21cip1. (B) Immunoprecipitations were performed with cyclin D2–specific antibody followed by immunoblot with a monoclonal antibody specific for p27kip1.

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