Fig. 3.
Core proteins of endothelial cell–secreted proteoglycans.
Digestions were performed as described in the text. The digestion products were separated by SDS-PAGE and visualized by autoradiography. Proteoglycans from the Sepharose CL-6B column were pooled in 2 fractions: the Vo peak (peak 1) and the entire Kav 0.2 peak (peak 2). Un indicates undigested; C, chondroitinase; H, heparitinase; C/H, double digestion with chondroitinase and heparitinase. Undigested material represents 10% of the sample, and each digest represents 30%.