Fig. 6.
Immunoblotting and autoradiographic detection of serglycin in HL-60 cells.
HL-60 cells were incubated with 35S-sulfate, and proteoglycans were purified and subjected to chondroitinase digestion as described in the text. The digests were electrophoresed on SDS-PAGE gradient gels in duplicate and transferred to nitrocellulose. One blot was immunostained with the antiserglycin IgY and the other with preimmune serum. The blots were subjected to autoradiography. The radioactive band presumably representing the serglycin core protein was coincident with the immunostaining on the blot treated with the antiserglycin IgY. The same results were obtained with CHRF-288-11 cells.
