![Fig. 1. Expression and functionality of CXCR4 on BM myeloma cells. / (A) Myeloma CD38hiCD45RA− BM cells and myeloma-derived cell lines were incubated with the anti-CXCR4 44.717.111 or control [C] P3X63 mAb and analyzed by flow cytometry. Results from 3 different CD38hiCD45RA− BM samples (upper panels) expressing low (left), medium (middle), or high (right) levels of CXCR4 is shown. (B) Myeloma CD38hiCD45RA− BM and NCI-H929 cells were preincubated without or with pertussis toxin (PTx) in DMEM/BSA 0.5% and allowed to migrate in Transwell chemotaxis chambers to medium alone or an SDF-1α–containing lower chamber. Results from 3 different MM-CD38hiCD45RA− BM samples expressing medium or high levels of CXCR4 are shown. Data represent the mean ± SD of duplicate samples. (C) Cells were incubated for the indicated times with SDF-1α (solid circles) or adhesion medium (open circles), stained with fluorescein isothiocyanate–phalloidin, and subjected to flow cytometry.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/2/10.1182_blood.v97.2.346/5/h80210597001.jpeg?Expires=1735555429&Signature=VMJI0SJUEO16XPlsk2mSr-wwhrZ6wVTvFYjrZZqoIlQX8pgeAu1xal9ksakwRscCLl9ikdNPMmIZIhpln8g51qMyVB8txV0a0EEsBSGEHNKM~fqCB-lbGFEopyEzhgDTosSLS4Yu86coBaTwFI-lMUHsjNMbFJXXZ2eCGuAA5f4~Y8MXs7kV6ditXWcD2uqkv9reGeW091vejd6JVOQvNUMhw7wRLcnrSuUAs4oMCtv12GRqKgvGvoxb8LhHSbvdZKWF7OXFpOUAQiecSIV~oiEpYHTFiYkqF5Mlgz2ROHkkncphCn2w-wHgZg5QZDHRSnfcw~4PsP~E1IhWibNwuw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression and functionality of CXCR4 on BM myeloma cells.
(A) Myeloma CD38hiCD45RA− BM cells and myeloma-derived cell lines were incubated with the anti-CXCR4 44.717.111 or control [C] P3X63 mAb and analyzed by flow cytometry. Results from 3 different CD38hiCD45RA− BM samples (upper panels) expressing low (left), medium (middle), or high (right) levels of CXCR4 is shown. (B) Myeloma CD38hiCD45RA− BM and NCI-H929 cells were preincubated without or with pertussis toxin (PTx) in DMEM/BSA 0.5% and allowed to migrate in Transwell chemotaxis chambers to medium alone or an SDF-1α–containing lower chamber. Results from 3 different MM-CD38hiCD45RA− BM samples expressing medium or high levels of CXCR4 are shown. Data represent the mean ± SD of duplicate samples. (C) Cells were incubated for the indicated times with SDF-1α (solid circles) or adhesion medium (open circles), stained with fluorescein isothiocyanate–phalloidin, and subjected to flow cytometry.
