Effect of SDF-1α on VLA-4–dependent myeloma cell adhesion.

BCECF-AM–labeled cells were preincubated in adhesion medium in the absence or in the presence of SDF-1α (100 ng/mL) for 5 or 30 minutes (A) or for the times indicated (NCI-H929 cells) (B) and were allowed to adhere to FN-H89– or sVCAM-1–coated wells for 2 minutes. Some samples were preincubated with the anti-α4 HP1/2 mAb before exposure to SDF-1α. Adhesions were quantified in a fluorescence analyzer. Adhesion data represent the mean ± SD of triplicate samples from one representative result of at least 4 independent experiments. Basal adhesion to wells coated with BSA alone is also shown. *Statistical significance compared with control and assessed asP < .05.