Fig. 2.
![Fig. 2. PKC assay. / PKC activity was assayed by quantitation of 32P incorporation from [γ32P]-ATP into the substrate, MARCKSPSD peptide, by using lysates from HMVEC-Ls treated with Tat for the indicated times. The 32P-incorporated substrates were precipitated with TCA plus BSA, and the amount of radioactivity in the precipitates was measured by Cherenkov counting.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/2/10.1182_blood.v97.2.352/5/h80210602002.jpeg?Expires=1735548359&Signature=4rzU3R9gpdcUbwN7ClCfE8iJVbqjIhDw4LDHRklLIVElGMq8iPTKSDK0FfqfpsFUfZkJC0jUN71XjohS2kmMF02OECyq43B1Q4WMsq8rQhzZFch~w7Rjpq4eAPJQ-C9lLG6WZfIqmmKWFjsMFEG7pJGS7hUB3-YyEjyeEFg-X8isBXtpY~osfIM~x18OhUnl7pRJ3sb3uXZkM0G9vabhsfJB9nNAphC1883i0cWL4260IL4H5A6LzmM5ms6pudoz4L4juEyPmN8GIg7HuWjV~s2eV2Wjr8PtPpKfNC7vfXPmeVauHyC3380abLwInBplnanqUW6tHVeAMlxnjsoNow__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PKC assay.
PKC activity was assayed by quantitation of 32P incorporation from [γ32P]-ATP into the substrate, MARCKSPSD peptide, by using lysates from HMVEC-Ls treated with Tat for the indicated times. The 32P-incorporated substrates were precipitated with TCA plus BSA, and the amount of radioactivity in the precipitates was measured by Cherenkov counting.
