Effect of kinase inhibitors on Tat-induced MCP-1 secretion.

Starved cells were incubated with the indicated amounts of kinase inhibitors in EBM-2 medium 1 hour before stimulation with Tat. MCP-1 levels in the clarified culture supernatants were then quantitated as described in “Materials and methods.” PD and GFX indicate PD98059 (MAP kinase inhibitor) and GF109203X (protein kinase C inhibitor), respectively.