Chemotactic activity of culture supernatants from Tat-treated HMVEC-Ls.

Migration of the THP-1 cells (A) and CD14⁺ monocytes (B) was determined as described in “Materials and methods.” The chemoattractants placed onto the upper/bottom chambers were as follows: (1) EBM-2/EBM-2; (2) EBM-2/MCP-1; (3) EBM-2/untreated culture supernatant; (4) EBM-2/Tat-treated culture supernatant; (5) MCP-1/Tat-treated culture supernatant; and (6) Tat-treated culture supernatant/Tat-treated culture supernatant.