Expression of

hiwi in subpopulations of CD34⁺ cells.Immunomagnetically selected CD34⁺ human adult BM cells were sorted based on the CD38 expression. (A) Gates were established for the flow cytometric isolation of CD34⁺CD38^hi, CD34⁺CD38^lo, and CD34⁺CD38^neg. (B) Semiquantitative RT-PCR ofhiwi in CD34⁺ subpopulations. Lane 1, 123-bp DNA ladder; lane 2, CD34⁺CD38^neg sample; lane 3, CD34⁺CD38^neg (−RT) sample; lane 4, CD34⁺CD38^lo sample; lane 5, CD34⁺CD38^lo (−RT) sample; lane 6, CD34⁺CD38^hi sample; lane 7, CD34⁺CD38^hi (−RT) sample; lane 8, negative control (water); lane 9, positive control (human testis, 269 bp).