Fig. 4.
Expression of
hiwi in human leukemia cell lines. (A) Lane 1, 1-kb ladder; lane 2, TF-1; lane 3, Jurkat; lane 4, KG1a; lane 5, KG1; lane 6, K562; lane 7, CEM; lane 8, BV173; lane 9,SUPB13; lane 10, negative control (water); lane 11, human testis positive control (2.3 kb). (B) Samples without reverse transcriptase; same setup as panel A, excluding lanes 7 through 11 (samples 7-9 were donated cDNAs and total RNA samples were not available to perform the −RT control). (C) GAPD internal control (142 bp); lane numberings correspond to the same samples as in panel A, excluding lanes 10 and 11.