Histopathology of neonatal and adult

sph^Dem/sph^Dem mice.Histological sections of heart, liver, and kidney fromsph/sph and sph^Dem/sph^Demmice. Bars = 20 μm. (A, C, E, G, H): sph/sph. (B, D, F, I, J, K, L): sph^Dem/sph^Dem. (A,B): Heart sections of neonates stained with H&E. Note the presence of thrombi (indicated by arrows) in the valve and atrium of (B). (C,D): Liver sections from neonates stained with H&E. Note the presence of an infarcted region (identified by arrows) in (D). (E,F): Kidney sections from adult mice, stained with Gomori iron stain, which stains nonhemoglobin iron blue. (G,I,K): Liver sections from adult mice stained with H&E. Clusters of extramedullary hematopoiesis (arrowheads) stain purple. Arrows in (I) identify a large calcified lesion indicative of earlier infarction. (H,J,L): Identical regions of liver as shown in (G,I,K), stained with Gomori iron stain.