Effect of A2/C2-BAR CD8 T cells on naïve B cells. (A) Significant loss of IgM⁺ FVIII-specific spot formation with LPS stimulation of naïve B cells in A2/C2-BAR–treated group compared with control OVA-BAR or no T-cell groups (P = .0209). (B) Dose-dependent effect of A2-BAR, C2-BAR, or A2/C2-BAR CD8 T cells on the LPS-stimulated naïve B cells to form IgM⁺ FVIII-specific spots compared with the OVA-BAR group (P = .001). (C) TNP-specific IgM (top) or OVA-specific IgM (bottom) secretion by the LPS-stimulated naïve B cells in the presence of either OVA- or A2/C2-BAR–expressing T cells.