Accentuated pulmonary inflammatory and coagulation responses in lungs of bleomycin-injured PN-1^−/−mice.PN-1^−/− and their PN-1^+/+ littermates were subjected to bleomycin-induced lung injury (BLM; 2 mg/kg) or physiological serum (sham) by intratracheal instillation for 4 to 5 days. BALFs were collected from lung tissues and the number of WBCs (A), platelets (B), and red blood cells (RBCs) (C) were determined by counting in the hemocytometer. Thrombin activity was measured by the fluorometric method (D) and D-dimer by enzyme-linked immunosorbent assay (E). Data (mean ± standard error of the mean [SEM]; n = 6-8 per group) were analyzed by 1-way analysis of variance with a Tukey’s multiple comparison test. *P < .05, **P < .01, ***P < .001, ****P < .0001 vs respective control. ns, not significant.