American Society of Hematology

Figure 6.

$Figure 6. Interaction between recombinant VWF and recombinant SDC-1 exposed by HEK293 cells. (A) Fluorescence microscopic images of cells expressing human rhSDC-1 fused to the red fluorescent protein DsRed (SDC-1+) were incubated with or without 20 µg/mL of enhanced green fluorescent protein (eGFP)–labeled human VWF. HEK293 cells that were transfected with an EV served as a control. Scale bar, 10 µm. Inset shows the colocalization between the VWF and membranous SDC-1. (B) Flow cytometric measurement of the dose-dependent binding of VWF to SDC-1+ and EV cells (n = 3). (C) Binding of VWF to HEK cells in the presence of unfractionated heparin (UFH) or after manipulation of the glycocalyx with heparinase (hep’nase) and/or COS (n = 3-9). **P ≤ .01 (1-way analysis of variance and Bonferroni post hoc test).$

Interaction between recombinant VWF and recombinant SDC-1 exposed by HEK293 cells. (A) Fluorescence microscopic images of cells expressing human rhSDC-1 fused to the red fluorescent protein DsRed (SDC-1⁺) were incubated with or without 20 µg/mL of enhanced green fluorescent protein (eGFP)–labeled human VWF. HEK293 cells that were transfected with an EV served as a control. Scale bar, 10 µm. Inset shows the colocalization between the VWF and membranous SDC-1. (B) Flow cytometric measurement of the dose-dependent binding of VWF to SDC-1⁺ and EV cells (n = 3). (C) Binding of VWF to HEK cells in the presence of unfractionated heparin (UFH) or after manipulation of the glycocalyx with heparinase (hep’nase) and/or COS (n = 3-9). **P ≤ .01 (1-way analysis of variance and Bonferroni post hoc test).

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