Lung and liver pathology and lung dysfunction in B6→B10.BR mouse model of cGVHD. (A) Collagen deposition (blue staining) in lung and liver of control animals (left column) and cGVHD animals (right column) (Masson’s trichrome staining, Nikon Eclipse Ti microscope, original magnification ×200). (B) Arterial oxygen saturation was measured using pulse oximetry in cGVHD animals; ***P < .001 (Mann-Whitney U test); n = 15 and n = 12 for the BM-only and BM plus T cells groups, respectively. Bars represent standard error of the mean. (C) Lymphocyte infiltration in the liver of control animals (top panel) and cGVHD animals (bottom panel) (hematoxylin and eosin staining, bright-field microscopy using Nikon Eclipse Ti microscope, original magnification ×100).