Comparison of cGVHD model and high T-cell dose B6->B10.BR model that mimics aGVHD. B10.BR mice were transplanted with BM only or BM and either a low (5 × 10⁴) or high (5 × 10⁵) dose of T cells from B6 donor mice. Tissues from the animals were harvested on day 17. (A) Levels of TNF-α and IFN-γ in the small intestine of the mice 17 days posttransplant. ***P < .001 (Mann-Whitney U test); n = 12 and n = 9 for cGVHD and aGVHD groups, respectively. Bars represent standard error of the mean. (B) Lymphocyte infiltration in the submucosa of the small intestine of cGVHD (top panel) and aGVHD (bottom panel) animals (hematoxylin and eosin staining, bright-field microscopy using a Nikon Eclipse Ti microscope, original magnification ×100).