Concentration of EV and subpopulation in RCC products stored for up to 42 days analyzed by the TRPS and flow cytometry systems. (A) EV < 200 nm, (B) EV ≥ 200 nm, (C) total EV, (D) RBC-EV, (E) platelet-EV, and (F) WBC-EV. Data are reported as mean ± standard deviation. *Significant results (P < .05) in comparison with day 5 values. ^δSignificant difference (P < .05) compared with the noted blood manufacturing methods (n = 8 per blood manufacturing method).