Imetelstat treatment has modest effects on normal SRCs. (A) Representative fluorescence-activated cell sorter (FACS) plots showing hCD45 cell chimerism and hCD34⁺ cells generated in the marrow of NSG mice receiving the same CB CD34⁺ cells treated with vehicle alone, MM, or Ime (15 mg/kg). (B-E) The absolute number of hCD45⁺ and hCD34⁺ cells generated in the marrow of NSG mice 4 months after the transplantation of normal CB CD34⁺ cells. One week after the transplantation, the recipient mice were treated with vehicle alone, MM (5-30 mg/kg), or Ime (5-30 mg/kg) for 4 weeks (B-C) or 8 weeks (D-E). The absolute number of hCD45⁺ and hCD34⁺ cells was calculated by multiplying the total number of marrow cells (cells harvested from 2 tibias, 2 femurs and 2 humeri) by the percent of hCD45⁺ and hCD34⁺ cells, respectively. The data were presented as the percentage of the absolute number of hCD45⁺ and hCD34⁺ cells present in the marrow of recipient mice treated with MM or Ime relative to that detected in the mice treated with vehicle alone. Three samples of pooled CD34⁺ cells from 8 to 10 CB donors were transplanted. P all > .05, MM vs Ime at each dose. Two independent experiments were performed for each sample.