FeCl3-induced carotid artery thrombosis model. The common carotid artery of anesthetized mice was exposed to (A) 8% or (B) 9% FeCl3, and blood flow was monitored for 30 minutes afterward. Time to first occlusion is defined as time recorded until the blood flow rate decreased to <0.1 mL/min; time to stable occlusion is defined as time until flow <0.1 mL/min was recorded for at least 10 minutes. Flow index is the ratio between the recorded blood volume flowing through the artery in 30 minutes and that calculated assuming a constant flow at the maximum rate recorded during the first minute after injury. Arteries that did not occlude were reported as time to first and stable occlusion >2000 seconds. (A) M1MA, n = 9; M1HA, n = 6; H1MA, n = 9; H1HA, n = 9. (B) M1MA, n = 6; M1HA, n = 6; H1MA, n = 7; H1HA, n = 6. Data are shown with median and interquartile range. Statistical analysis was performed with the Kruskal-Wallis nonparametric test followed by Dunn’s multiple comparison test for first and stable occlusion and by ordinary one-way ANOVA followed by Tukey’s multiple comparison test for flow index. *P < .05; **P < .01; ***P < .001.