Accelerated LTI is more effective than conventional LTI in the induction of A20 lymphoma cell necrosis with expression of CRT, Hsp70, and Hsp90. (A) Gated CD19⁺ lymphoma cells from untreated or treated A20 tumors, 24 hours after completion of LTI, were analyzed for 7AAD vs annexin V staining. Gates were drawn based on fluorescence minus 1 controls. (B) Representative 1-color analyses of intensity of staining with CRT, Hsp70, or Hsp90 among gated CD19⁺ lymphoma cells that are Ann^lo7AAD^neg (box 1 in panel A, red), Ann^int7AAD^lo (box 2 in panel A, blue), and Ann^hi7AAD^hi (box 3 in panel A, green). (C) Percentages (mean ± standard error of the mean) of cells in boxes 1, 2, and 3 in panel A. (D) Mean percentages of CRT⁺, Hsp70⁺, or Hsp90⁺ tumor cells in boxes 1, 2, and 3 in panel A in the 4 groups of mice (n = 5 mice per group). Mean percentages of early apoptotic cells in all groups were significantly different from those in late-apoptotic and necrotic cells. Only significant P values are shown. *P < .05, **P < .01, Mann-Whitney U test.