MRTFA^OEin HEL cells increases ploidy. (A) Western blot shows Dox-inducible MRTFA^OE. Cells were either left untreated (control) or induced for 24 hours with 15 nM TPA, 10 ng/mL Dox, or both Dox and TPA. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. (B) Same cells have significantly higher 8N and 16N ploidy states when treated with Dox and TPA for 4 days (n = 3). Asterisks represent significant differences (P ≤ .001) from TPA-induced cells with no Dox.