Molecular determination of sickle cell, α-thalassemia, and G6PD status. Each panel represents example allelic discrimination (A,C) or probe amplification (B) plots for the reverse transcriptase-PCR assays used to determine each blood disorder. (A) Molecular determination of the presence of absence of the HbS mutation. (B) Probe amplification plots by α-globin genotype, demonstrating later probe amplification with each α-globin gene deletion. (C) Allelic discrimination plot to determine G6PD A vs B isoforms, the presence or absence of the A⁻ isoform, and a confirmatory sex assay to identify male and female samples.