Condensed and decondensed α-granules distribution remains predominantly central during shape change in stimulated platelets. Each row shows various views of the same platelet. (A,D,G) Individual slices from SBF-SEM image stacks showing changes in platelets at various times of stimulation. Blue arrows indicate condensed granules; tan/yellow arrows indicate decondensed granules. (B,E,H) Condensed granules are rendered in blue, and decondensed granules in tan/yellow. (C,F,I) PM is rendered in green. A decondensed granule appeared to be connected to the PM via a long pipe as shown in panel F. Supplemental Figure 3 Movie shows the 90 seconds rendered example rotated in 3-dimensional space. α-Granule surface area and sizes were quantified from similar renderings for multiple randomly chosen platelets (see Figure 4 for additional examples), and the quantitation of their characteristics is reported in Tables 1 and 2.