Time course of expression of TM and E-selectin. (A-E) Immunohistochemical staining for TM on blood vessels (arrowheads) in skin biopsy specimens taken at various time points during the patient’s hospitalization (days 1-28) and at convalescence (day 231) compared with a normal control specimen (scale bars, 20 µm). Staining for E-selectin, a marker of endothelial cell activation (F-J), and for ICAM-1, a marker of cell viability (K-O), in the same biopsy specimens. (P) Quantification of the immunohistochemical staining of TM, E-selectin, and ICAM-1 in (A-O), represented as fold change from day 0. Expression of TM and E-selectin returned to baseline at day 231. (Q) TM and E-selectin transcript levels normalized to GAPDH expression at each time point, as determined by real-time quantitative PCR. (R) Quantification of the patient’s convalescent TM and E-selectin transcript levels as a ratio of the transcript levels in a healthy subject.