Figure 4.
Bone marrow transplantation of E84-high and E84-low mouse LSK cells. (A) Schematic of bone marrow transplantation. Two hundred sorted E84-high or E84-low CD45.1 LSK cells were transplanted along with 2 × 105 CD45.2 whole bone marrow cells into lethally irradiated mice (E84-low, n = 7; E84-high, n = 8). (B) Percentage of CD45.1 cells of different lineages in peripheral blood over the course of 4 months. (C) Percentage of CD45.1 cells in different lineages in recipient mouse bone marrow at 16 weeks posttransplantation. Lineage-specific surface markers: Mac-1/Gr-1 for myeloid lineage; B220 for B cell lineage, and CD3 for T cell lineage. The data are shown as mean ± standard deviation. *P < .05; **P < .01; ***P < .001; ****P < .0001.