CD16⁺and CD16⁻MDDCs exposed to media, LPS, or HIV exhibit unique molecular signatures. Genome-wide transcriptional profiles were generated as described in Figure 1A,D-H for MDDC subsets exposed to media, LPS, or HIV for 24 hours. (A) Gene set enrichment analysis allowed the identification of top-regulated canonical pathways (C2), commonly and differentially expressed between CD16⁺ vs CD16⁻ MDDCs exposed to media, LPS, or HIV-1. (B) Shown are top-regulated micro-RNAs (MIR) differentially expressed between CD16⁺ and CD16⁻ MDDCs exposed to media, LPS, or infectious HIV virions. Results were generated with matched MDDC subsets from 5 different donors.