Hyperlipidemic apoE-null animals with CD36 or ERK5 deficiency showed decreased fibrin accumulation in vivo. ApoE-null or apoE:CD36 double-null mice at 8 weeks of age were fed a control or high-fat diet for at least 6 weeks before performing laser-induced in vivo arterial thrombosis on the cremasteric artery (A-C). Video microscopy of the platelet accumulation (in green) and fibrin accumulation (in red) in real time up to 5 minutes is shown in panel A; the arrow indicates the direction of arterial flow and the side the arrow is on indicates the side of vessel injury. Quantification of fibrin (B) and quantification of platelets (C). ApoE-null mice at 8 weeks of age were irradiated and were transplanted with bone marrow from ERK5^flox/flox or ERK5^flox/flox:PF4-cre+ mice. (D-E) After 2 weeks of recovery, the apoE-null chimeric mice were put on chow or a high-fat diet for at least 6 weeks before performing the adventitial tissue-mediated in vivo arterial thrombosis on the carotid artery (supplemental Figure 2). The quantification of peak fibrin accumulation with this model is shown in panel E by using the 1-site nonlinear curve fitting model. Video microscopy images of fibrin accumulation in real time are shown in supplemental Figure 2. N of >5 age-matched male mice per group in panels A-C; >6 age-matched male or female mice per group in panels D-E.