Genome editing of nfe2 results in a frameshift mutation and early stop codon. (A) Alignment of human and zebrafish Nfe2 protein sequences. Sequences are highly conserved in the CNC (blue), basic DNA binding (green), and leucine zipper domains (pink). The site of the deletion mutation and beginning of the frameshift is marked in yellow. (B) Sequencing of homozygous mutant offspring cDNA demonstrates the 8-bp deletion compared with wild-type siblings. (C) Survival of nfe2 mutant fish into adulthood is not significantly different when compared with wild-type siblings (P = .3, log rank [Mantel-Cox] test).