Reduction of circulating thrombocytes in adult but not larval nfe2^−/−fish. (A) Peripheral blood (PB) samples from 2.5-month postfertilization adult Tg(cd41:egfp) fish show a significant decrease in GFP^high thrombocytes in nfe2^−/− fish as compared with nfe2^+/+ siblings (green data points; P < .0001). The numbers of GFP^high and GFP^low cells in kidney marrow (KM) samples (purple and blue, respectively) are significantly increased between nfe2^−/− and nfe2^+/+ fish (P = .0003 and P = .0001, respectively). (B) Circulating thrombocyte numbers in 6-dpf larval fish are unchanged between homozygous mutants and either wild-type or heterozygous siblings (P = .86 and P = .33, respectively), or between heterozygous and wild-type siblings (P = .38). (C) Circulating thrombocyte numbers in 18-dpf larval fish were also unchanged between homozygous mutants and either wild-type or heterozygous siblings (P = .12 and P = .39, respectively), or between heterozygous and wild-type siblings (P = .42). Bars indicate the mean and SD (A) or median (B-C). N.S., nonsignificant.