Production of miR-146a and miR-146b KO mice with TALEN. (A) Genetic deletion in miR-146a– and miR-146b–deficient mice generated by TALEN. The genomic sequence is shown at the top, with the target sequences of a pair of TALEN colored in red. Blue lines and boxes indicate pre-miRNA and mature miRNA sequences, respectively. Deleted nucleotides are indicated with dashes. The sizes of the deletions and insertions are shown to the right of the mutated alleles with Δ. WT indicates alleles without a mutation. (B) RT-PCR analysis of miR-146a and miR-146b in liver tissue derived from mutant mice. The genotype of each animal is indicated at the bottom. The expression level of miR-146a and miR-146b in the WT animal was adjusted to 1.