DNMT3A mutations at diagnosis are associated with NPM1^wtrelapse. Cooccurring mutations with NPM1 at diagnosis of all patients. For 61/90 patients with NPM1^mut relapse and for 11/14 patients with NPM1^wt relapse, the diagnostic sample was available for 63 gene panel sequencing. Shown are mutations in genes affected in >10% of cases. DNMT3A mutations are significantly more frequent in patients that relapse with NPM1^wt disease. FLT3-ITD mutations are significantly more frequent in patients that relapse with NPM1^mut disease. n.a., not available.