Plasminogen deficiency did not alleviate the bleeding phenotype or enhance the response to rFVIII treatment in F8^−/−mice. (A) Graphical representation of individual bleeding profiles of mice treated with Advate buffer or rFVIII at 2.5 and 3.0 U/kg. Each row represents the entire bleeding profile of a single mouse with each bar representing a single bleeding episode for that mouse. The number of spontaneous bleeds (B), total blood loss (C), and bleeding time (D) of F8^−/− and F8^−/−/Plg^−/− mice were quantified. Data are presented as the mean ± SEM and analyzed by 2-way ANOVA applying the Bonferroni test to adjust for multiple comparisons. *P < .05, **P < .01, ***P < .001, ****P < .0001. hgb, hemoglobin.