Infection and replication of MV depends on CD46 expression. (A) BCN and LP1 cells were incubated or not with a blocking anti-CD46 mAb (final concentration of 10 μg/mL) for 1 hour prior to infection with MV-GFP. Fluorescence was assessed after 3 days by flow cytometry. Cell death was assessed by the percentage of cells excluded from the living gate. (B) BCN and LP1 cells were transfected with siControl (siCont) or siCD46. On day 1, CD46 expression was assessed by flow cytometry and MV-GFP (MOI = 1) was added to the cultures (no removal of siRNAs). GFP expression was assessed 48 hours after MV-GFP addition. Viability is expressed as the percentage of cells in the living gate. The histograms represent the GFP fluorescence in the total population. One representative experiment (triplicate wells) out of 2 is shown. CD46 histograms: thin line, control staining; thick line, CD46 staining. GFP histograms: thin line, noninfected cells; thick line, MV-GFP infected cells. Statistical analyses were performed using the paired Student t test. *P < .05; **P < .01; ***P < .001. ns, not significant.