Induction of human LIN28B in adult mice reduces platelet P-selectin transcript and cell surface expression. (A) Integrin activation in unactivated and thrombin-activated platelets from mice with doxycycline-induced expression of LIN28B (iLIN28B) and control mice. (B) Surface expression of P-selectin (CD62P) in thrombin-activated platelets from iLIN28B and control mice. Significance was determined using a 2-way ANOVA followed by a Bonferroni posttest ***P < .001; n ≥ 6. (C) Transcript levels of human LIN28B and murine Selp relative to murine PF4 in cultured megakaryocytes from mice with doxycycline-induced expression of human LIN28B (iLIN28B) and control mice. Significance was determined using 2-way ANOVA. *P < .05; **P < .005; n = 3.