SNVs at CREs are associated with TPRG1 expression in DLBCL. (A) SNVs at a CRE interacting with the TPRG1 promoter. The top panel shows the position of SNVs at the CRE. The second panel shows chromatin looping interactions between the TPRG1 promoter and CREs, with the interaction between the promoter and the SNV-disrupted CRE colored yellow. The third panel details chromatin immunoprecipitation–sequencing histone mark signals in naive B cells. The bottom panel illustrates positions of BRD4-bound enhancers in DLBCL.³⁵  (B) CRE mutation status and gene expression. Although the TPRG1 CRE is mutated in 9 DLBCL tumors, only 6 tumors were considered in the differential expression analysis, as 3 tumors have CNVs at the CRE or target gene. Differential expression assessed by using t value permutation test. Boxplot hinges extend to the most extreme data points that are no more than 1.5 times the interquartile range from the box. RPKM, reads per kilobase per million mapped reads.