MYC sensitizes DLBCL to TAK-243-induced apoptosis. (A-B) U-2932 were treated with TAK-243 as shown. MYC protein and RNA levels were evaluated by immunoblotting and RT-PCR. (C) OCI-LY3 cells were engineered to express MYC or vector control. MYC overexpression was confirmed by RT-PCR. Cells were treated with 300 nM TAK-243 for 24 hours, and apoptosis was assessed by Annexin V staining. (D) OCI-LY19 cells were engineered to express shMYC or vector control. MYC knockdown was confirmed by RT-PCR. Cells were treated with 300 nM TAK-243 for 24 hours, and apoptosis was assessed by Annexin V staining. (E-F) Cells manipulated to express MYC or shMYC were treated with 300 nM TAK-243 for 4 hours. Proteins were lysed and subjected to immunoblotting. Expression of ER stress genes was assessed by RT-PCR with the indicated probes. (G) OCI-LY19 cells were treated with SKT and TAK-243 for 4 hours. Proteins were lysed and subjected to immunoblotting. (H) OCI-LY19 cells were treated with SKT and TAK-243 for 24 hours. Apoptosis was assessed by Annexin V staining. Data are presented as mean ± SE. *P < .05; **P < .01.