IL-10R blocking is correlated to elevated TNF-α. (A) Normal monocytes (n = 2 PV, 2 ET, and 1 MF) were stimulated with 10 ng/mL LPS and with the addition of 1 μg/mL anti-IL-10R. Supernatants were collected for TNF-α quantification by ELISA at 4, 9, 18, and 24 hours after LPS stimulation. The amount of TNF-α produced at 4 hours was normalized to 1. (B) The correlation of TNF-α persistence score in MPN monocytes (n = 14) is defined as (TNF-α at 24 hours)/(TNF-α at 4 hours), and IL-10 resistance score is defined as (TNF-α of LPS + 1 ng/mL IL-10)/(TNF-α of LPS + 0 ng/mL IL-10). Pearson r = 0.7198, R² = 0.5181. *P < .05.