Neutrophil-derived OSM increases P-selectin–dependent thrombosis in flow-restricted veins. (A-B) Quantification of endothelial surface area covered with firmly adherent Ly6G⁺ neutrophils or M-CSFR⁺ monocytes using spinning-disk intravital microscopy, 3 hours after ligation of the IVC in mice of the indicated genotype. (C-D) Kinetics of thrombus development (frequency) and thrombus size (area) in the indicated genotype, measured by ultrasonography at the indicated times after ligation. (E) Thrombus weight in the indicated genotype 24 hours after ligation. Each symbol represents an individual thrombus. Horizontal red bars represent median values. (F-G) Number of Ly6G⁺ neutrophils or M-CSFR⁺ monocytes per thrombus 24 hours after ligation in the indicated genotype, as measured by flow cytometry. (H-I) Normalized thrombus weight per Ly6G⁺ neutrophil or M-CSFR⁺ monocyte. (J) Western blot of thrombus lysates probed with antibodies to fibrin, Ly6G, and citrullinated histone. (K) Western blot of thrombus lysates probed with antibodies to fibrin, M-CSFR, and tissue factor. The Aα, Bβ, and γ chains of fibrin are marked. The data in panels A-I represent the mean ± SEM from 5 to 12 mice in each group. The data in panels J-K are representative of 3 experiments. *P < .05; **P < .01.