Figure 1.
Figure 1. ELMO1 associates with PIP3 in platelets. (A) Resting human platelets (1 × 109 cells/mL) were lysed with NP-40 lysis buffer and incubated with PI(3,4,5)P3 PIP beads or control beads overnight at 4°C. Pull-downs were analyzed by western blot and probed with ELMO1 or Btk antibody. Blots are representative of at least 3 independent experiments. (B) The proteins from resting washed human, WT littermate control, and ELMO1−/− murine platelets were precipitated and analyzed by western blot. The blots were probed for ELMO1 and β-actin as a loading control. Blots are representative of at least 3 independent experiments. MW, molecular weight marker.

ELMO1 associates with PIP3in platelets. (A) Resting human platelets (1 × 109 cells/mL) were lysed with NP-40 lysis buffer and incubated with PI(3,4,5)P3 PIP beads or control beads overnight at 4°C. Pull-downs were analyzed by western blot and probed with ELMO1 or Btk antibody. Blots are representative of at least 3 independent experiments. (B) The proteins from resting washed human, WT littermate control, and ELMO1−/− murine platelets were precipitated and analyzed by western blot. The blots were probed for ELMO1 and β-actin as a loading control. Blots are representative of at least 3 independent experiments. MW, molecular weight marker.

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