Enhanced thromboxane generation in response to GPVI agonists in ELMO1^−/−platelets. (A) Washed murine platelets were activated with the indicated concentrations of CRP or collagen for 4 minutes, and the reaction was stopped via flash-freezing. Thromboxane B2 generation was determined using an ELISA kit as per the manufacturer’s instructions. Data are presented as mean ± SE of at least 3 independent experiments and were analyzed by Student t test (*P < .05). (B) Representative aggregation tracings and dense granule secretion of washed platelets from ELMO1^−/− or WT littermate controls preincubated with the feedback inhibitors (FBI’s) 10 μM indomethacin, 10 μM MRS-2179, and 100 nM AR-C69931MX for 5 minutes and activated with indicated concentration of CRP for 3 minutes. (C) Quantitation of aggregation and ATP secretion showing the mean ± standard error of the mean and analyzed by Student t test (*P < .05).