Method and cell population determination. (A) Illustration of method. (B) Example of flow cytometry gating of skin and PBMC samples. (C) Plot of T-cell constituent populations in clonotypic samples. In the penultimate 3 samples, the clonotypic population represented >40% of the CD4^low (DNEG) population; in the last sample, the clonotype was >40% of the PB CD4⁺ cells. (D) Mean forward and side scatter of the 5 analyzed populations.