Knocking down Il34 expression in MOPC315.BM cells. (A) Knockdown efficiency of Il34 expression in MOPC315.BM cells that stably express Il34-specific siRNA compared with control as evaluated by qRT-PCR (control = 100%). (B) Cellular morphology of MOPC315.BM^Control (top: hematoxylin and eosin stain; original magnification ×20) or MOPC315.BM^Il34KD (bottom: hematoxylin and eosin stain; original magnification ×20) cells. (C) Cell proliferation as compared between MOPC315.BM^Control and MOPC315.BM^Il34KD cells. (D) ELISA measurement of M315 protein in the supernatants of MOPC315.BM^Control or MOPC315.BM^Il34KD cell cultures. (E) qRT-PCR analysis of Il34 expression in MOPC315.BM^Control or MOPC315.BM^Il34KD cells collected from bones of mice (n = 3) at day 30 or day 45 postinjection (normalized to Gapdh). (F-G) Bioluminescence imaging in representative BALB/c mice challenged with MOPC315.BM^Control (F) or MOPC315.BM^Il34KD cells (G) at day 45 postinjection. Ventral and dorsal views are shown in the top and bottom panels, respectively. Data are mean ± SEM; *P < .05. OD, optical density.