American Society of Hematology

Figure 2.

$Figure 2. RORγt expression identifies MAIT cells as the predominate potential IL-17A–producing population after allogeneic SCT. (A) Gating strategy employed to identify MAIT (CD3+CD161+Vα7.2TCR+), natural killer T (CD3+CD1d tetramer+), and γδT (CD3+γδTCR+) cells in unfractionated recipient PBMCs. (B) Representative contour plots generated by gating on the total CD8+ T-cell population depicting RORγt and Tbet expression compared with isotype control mAb–stained PBMCs. (C) Frequency of RORγt expression within T-cell populations derived from unfractionated recipient PBMCs at days +30 (n = 15) and +180 (n = 15) posttransplant. (D) Representative contour plots generated by gating on the total CD8+ T-cell population depicting RORγt and Tbet expression in unfractionated PBMCs. RORγt and Tbet expression by MAIT (red), γδT (green), and putative Tc17 (blue) cells is shown. (E) Frequency of RORγthi Tc17, MAIT, and γδT-cells at day +30 (n = 15) and +180 (n = 15) posttransplant. Data presented as mean ± standard error of the mean. *P = .02. TCR, T-cell receptor.$

RORγt expression identifies MAIT cells as the predominate potential IL-17A–producing population after allogeneic SCT. (A) Gating strategy employed to identify MAIT (CD3⁺CD161⁺Vα7.2TCR⁺), natural killer T (CD3⁺CD1d tetramer⁺), and γδT (CD3⁺γδTCR⁺) cells in unfractionated recipient PBMCs. (B) Representative contour plots generated by gating on the total CD8⁺ T-cell population depicting RORγt and Tbet expression compared with isotype control mAb–stained PBMCs. (C) Frequency of RORγt expression within T-cell populations derived from unfractionated recipient PBMCs at days +30 (n = 15) and +180 (n = 15) posttransplant. (D) Representative contour plots generated by gating on the total CD8⁺ T-cell population depicting RORγt and Tbet expression in unfractionated PBMCs. RORγt and Tbet expression by MAIT (red), γδT (green), and putative Tc17 (blue) cells is shown. (E) Frequency of RORγt^hi Tc17, MAIT, and γδT-cells at day +30 (n = 15) and +180 (n = 15) posttransplant. Data presented as mean ± standard error of the mean. *P = .02. TCR, T-cell receptor.

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