Treatment with AGS-16C3F decreased the number of ROSA^{KIT D816V-Gluc}cells in hematopoietic organs. Mice were euthanized 4 days after the last injection of AGS-16C3F. (A,C) Fluorescence-activated cell sorter analyses of the percentage of hCD45⁺/KIT⁺ in BM (A) and spleen (C). (B,D) Absolute numbers of neoplastic MCs in BM (B) and spleen (D), calculated by: total cell number in 1 femur and in total spleen × % of hCD45⁺/KIT⁺ cells in BM and spleen. (E) The femurs from 4 mice were collected and stained immunohistochemically with anti-human CD45 Ab. The sections were counterstained with hematoxylin. *P < .05; **P < .01.