NCoR1 is essential for lymphoid differentiation but dispensable for erythropoiesis. (A) PB cell counts in 8-week-old control mice (n = 6) and Vav-iCre⁺, NCoR1^f/f mice (n = 5) determined using a Hemavet 950FS analyzer (Drew Scientific). (B) Fluorescence-activated cell sorting (FACS) analysis of T cells (CD3⁺), B cells (B220⁺), and myeloid cells (CD11b⁺) in PB samples from control mice (n = 6) and Vav-iCre⁺, NCoR1^f/f mice (n = 5). Representative FACS profiles (upper panel) and the relative frequency (lower left panel) and cell number (lower right panel) are shown. (C) FACS analysis of immature erythrocytes (CD71⁺Ter119⁺) in control and Vav-iCre⁺, NCoR1^f/f (n = 6 per group) PB samples. Representative FACS profiles (upper panel) and the relative frequency (lower panel) are shown. FACS analysis of CD71/Ter119 erythroid subsets in spleen (D) and BM (E) from control and Vav-iCre⁺, NCoR1^f/f mice (n = 5 per genotype). Representative FACS profiles (upper panels) and the absolute cell numbers of ProE (CD71^highTer119^intermediate), EryA (CD71^highTer119^highFSC^high), EryB (CD71^highTer119^highFSC^low), and EryC (CD71^lowTer119^highFSC^low) (lower panels) are shown. Data are mean ± standard error of the mean from 3 independent experiments. *P < .05, **P < .01, ***P < .001. Hb, hemoglobin; HCT, hematocrit; LY, lymphocyte; MCH, mean corpuscular hemoglobin; MCHC, mean cell hemoglobin concentration; MCV, mean corpuscular volume; MO, monocyte; MPV, mean platelet volume; NE, neutrophil; RDW, red cell distribution width.